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The GenScript Ni-Charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions. The GenScript Ni-Charged MagBeads are average 40 μm in size, superparamagnetic beads with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with nickel and ready to use for quick and small-scale purification of polyhistidine-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Ni-Charged MagBeads have a binding capacity of 5 to 20 mg 6xHis-tagged protein per 1 ml settled beads (e.g. 4 ml 25% slurry).